ABSTRACT
Objective To investigate the content changes of two alkaloid before and after the processing of Aconitumvilmorinianum and its effect on cardiotoxicity. MethodsThe UPLC method was used to determine the content of yunaconitine and 8-acetylacetonarine before and after the processing of A.vilmorinianum. The half lethal dose (LD50) of A.vilmorinianum to rats was determined by Bliss method. Rats wereig administrated A.vilmoriniauum and its processed products for 14 d. The chages of II lead electrocardiogram of anesthetized rats and myocardial morphology were observed,and the serum levels of lactate kinase (CK) and lactate dehydrogenase (LDH)were detected. Results The content of yunaconitine before and after the preparation of A.vilmorinianum was 263.96 μg/g and not detected respectively. The content of 8-acetylacetonarine was not detected and 568.47 μg/g respectively. The LD50 of the raw product was crude drug4.2 g/kg, and the maximum dose of the processed product was crude drug18.0 g/kg; In the anesthetized rats of the raw group, ventricular premature beats, ventricular bipolar law, and ventricular fibrillation occurred successively. The processed product group showed bradycardia in the early stage and returned to normal in 60 min; Compared with the blank group, the LDH and CK levels in the raw group were significantly increased (P< 0.05), and only the LDH level was significantly increased in the processed product group (P< 0.05); The gap between myocardial fibers in the raw group was significantly enlarged, and the infiltration of inflammatory cells was obvious. The myocardial fibers of the product group are arranged neatly, and individual inflammatory cells infiltrated in the interstitial. Conclusion At the given dose, A.vilmorinianum has greater cardiotoxicity, and the toxicity of A.vilmorinianum is obviously reduced after processing.